Advanta Immuno-Oncology Gene Expression Assay

The Advanta™ Immuno-Oncology Gene Expression Assay is a 170-gene expression qPCR assay that enables profiling of tumor immunobiology and new biomarker identification.

Designed to meet the rigorous demands of human checkpoint research programs, the Advanta Immuno-Oncology Gene Expression Assay includes 91 key markers of tumor immune response that were shown in a multicenter international clinical trial to inform tumor progression and checkpoint therapeutic response. In collaboration with leading researchers and biopharma, this panel was further expanded to include 74 additional immuno-oncology markers.

The complete panel set includes genes for identification and functional analysis of immune and cancer cells including markers found in defined T cell subsets, cytokines and chemokines and markers of immune regulation, immune cell fate and more.

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Immuno-Oncology Expression Assay

Panel A

Panel B

Housekeeping genes for panels A and B:
B2M, ACTB, GAPDH, GUSB, TFRC

Table 1. The Advanta Immuno-Oncology Gene Expression Assay panels A and B. This two panel set includes 91 target genes and 5 housekeeping genes on Panel A and 74 target genes and the same 5 housekeeping genes on Panel B. Panel B reserves 17 open assay inlets, so you have the added flexibility to add new assays over time for customization. Each panel will be available in packs of 2 or 10 (192 or 960 samples, respectively.

The Biomark HD advantage

The Advanta Immuno-Oncology Gene Expression Assay is optimized to run on the industry-proven Biomark HD system. This system utilizes integrated fluidic circuits (IFCs) to precisely combine multiple reactions at nanoliter volumes. Enabling you to easily run up to 96 samples at a time with the Advanta Immuno-Oncology Gene Expression Assay, the Biomark HD automated gene expression workflow delivers performance you can trust with limited hands-on time.

Figure 1. Integrated fluidic circuit (IFC). The Gene Expression 96.96 IFC accommodates up to 96 samples per run, with a total of 9,216 parallel reactions. This is the equivalent reaction number of 24 total 384-well plates.

Assay workflow

Figure 2. Assay workflow. The gene expression assay workflow uses the Juno™ system for IFC preparation and Biomark HD for qPCR thermal cycling and data acquisition. With only 30 minutes of hands-on time and four hours of total workflow time, you can run up to 96 samples using the GE 96.96 IFC, generating results for up to 9,216 PCR reactions.

Advanta Immuno-Oncology Gene Expression Assay ordering info

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For Research Use Only. Not for use in diagnostic procedures.