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Seminar | Unraveling Human Microglial Heterogeneity

Multiplexed Mass Cytometry Analysis of Human Microglia

Multiplexed Mass Cytometry Analysis of Human Microglia

Hear Chotima Böttcher, PhD, describe her investigation into the diversity and regional distribution of microglia, a specialized innate immune cell in the brain involved in neural development and neuropathology response.

In this talk recorded at the 7th Annual Mass Cytometry Summit in Prague, Böttcher explains how her lab overcame the limitations of studying human microglia and were able to resolve the heterogeneity of this unique cell, previously only demonstrated in the mouse brain.

Watch this video to hear about:

  • high-dimensional, large-scale immunophenotyping of post-mortem brain tissue with CyTOF® technology
  • development of a novel cryopreservation protocol in combination with barcoding for single-cell profiling of human microglia
  • identification of the phenotypic signature of human microglia in five different brain regions
  • comparative analysis of microglia with peripheral myeloid cells and cerebral spinal fluid


Chotima Bottcher

Chotima Böttcher, PhD
Senior Scientist and Principle Investigator Laboratory of Molecular Psychiatry
Charité, University Medicine Berlin

Don't miss out on the 8th Annual Fluidigm Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.

Register now

References

Böttcher, C. et al. “Human microglia regional heterogeneity and phenotypes determined by multiplexed single-cell mass cytometry.” Nature Neuroscience 22 (2018): 78–90.

For Research Use Only. Not for use in diagnostic procedures.