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Seminar | Mass Cytometry for Immune Profiling in Chronic Inflammation

Mass cytometry provides insights into chronic inflammation

New technical advancements coupled with immune profiling by mass cytometry provide insights into chronic inflammation

In support of research at DRFZ Berlin on immune pathogenesis in chronic inflammation and autoimmunity, Dr. Henrik Mei’s lab at the DRFZ Berlin have developed several technical improvements that enabled generation of high-quality mass cytometry data. Mei describes these innovations in this presentation given at the 7th Annual Mass Cytometry Summit in Prague.

In this live recorded video, Mei presents:

  • the development of dual fluorescent/metal-tagged antibodies that permit direct cross-platform comparisons
  • the use of silver nanoparticles for sensitive detection of weakly expressed antigens
  • the stabilization of cocktails of metal-labeled antibodies for longitudinal or multicenter immune phenotyping studies
  • the results of a systematic comparison between blood cells of rheumatoid arthritis subjects and matched controls


Henrik Mei

Henrik Mei, PhD
Scientific Head of Mass Cytometry
DRFZ Berlin, a Leibniz Institute

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References

Baumgart, S. et al. "Dual-labelled antibodies for flow and mass cytometry: a new tool for cross-platform comparison and enrichment of target cells for mass cytometry." European Journal of Immunology 47 (2017): 1,377–1,385.

Schulz, A.R. et al. “Silver nanoparticles for the detection of cell surface antigens in mass cytometry.” Cytometry A 91 (2017): 25–33.

For Research Use Only. Not for use in diagnostic procedures.