New technical advancements coupled with immune profiling by mass cytometry provide insights into chronic inflammation
In support of research at DRFZ Berlin on immune pathogenesis in chronic inflammation and autoimmunity, Dr. Henrik Mei’s lab at the DRFZ Berlin have developed several technical improvements that enabled generation of high-quality mass cytometry data. Mei describes these innovations in this presentation given at the 7th Annual Mass Cytometry Summit in Prague.
In this live recorded video, Mei presents:
- the development of dual fluorescent/metal-tagged antibodies that permit direct cross-platform comparisons
- the use of silver nanoparticles for sensitive detection of weakly expressed antigens
- the stabilization of cocktails of metal-labeled antibodies for longitudinal or multicenter immune phenotyping studies
- the results of a systematic comparison between blood cells of rheumatoid arthritis subjects and matched controls
Henrik Mei, PhD
Don't miss out on the 8th Annual Mass Cytometry Summit, taking place from June 20-21, 2019 in Vancouver, Canada.
Baumgart, S. et al. "Dual-labelled antibodies for flow and mass cytometry: a new tool for cross-platform comparison and enrichment of target cells for mass cytometry." European Journal of Immunology 47 (2017): 1,377–1,385.
For Research Use Only. Not for use in diagnostic procedures.