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CEL Seq

mRNA Sequencing

Tamar Hashimshony and Itai Yanai
Itai Yanai, Technion - Israel Institute of Technology
0.0
1

(...) Published on Rev B

  • supported ifcs:
    Open App IFC
  • number of ifc runs:
    6

Overview

This method employs 3'-end counting mRNA sequencing in vitro transcription for initial amplification on the C1 System. Adapted from Hashimshony, Wagner, Sher, & Yanai (2012) "CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification," Cell Reports 2:666-673. This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.

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Protocol: CEL Seq v1Duration (H:M): 18:00

Capture

CELseq

view protocol worksheet

Tested Primary Cells or Cell Lines

Cell Name Cell Type Source
Mouse fibroblasts Fibroblast Primary

Performance

Comparable to manual, tube based method.

Publications or Articles

Performance data

Not available

Additional documents

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