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CEL Seq 2

mRNA Sequencing

Tamar Hashimshony and Itai Yanai
Itai Yanai, Technion - Israel Institute of Technology
0.0
2

(...) Published on Rev A

  • supported ifcs:
    Open App IFC
  • number of ifc runs:
    6

Overview

CEL Seq 2 is a 3'-end counting mRNAseq method that uses in vitro transcription for initial amplification. CELseq2 incorporates numerous improvements over the original method including increased sensitivity and incorporation of unique molecular identifiers (UMIs). This script has been updated to be compatible with all versions of the C1 Open App IFCs, including the redesigned medium 96 cell IFC.

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Protocol: CEL Seq 2Duration (H:M): 18:00

Capture

CELseq

view protocol worksheet

Tested Primary Cells or Cell Lines

Cell Name Cell Type Source
primary mouse fibroblasts Primary

Performance

Publication documents increased number of genes detected per cell compared to manual method

Publications or Articles

Performance data

Not available

Additional documents

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Additional documents