Biomark HD / EP1高通量基因分析系统


1.  人群筛查 

Ÿ   Genetic variant of IL-10RA and susceptibility to rheumatoid arthritis in a Chinese population. Yang XK, Li P, Li S, Zhang C, Li BZ, Leng RX, Pan HF, Ye DQ. Clin Rheumatol. 2017, 36(4):825-830

Ÿ   Role played by the SP4 gene in schizophrenia and major depressive disorder in the Han Chinese population. Chen J, He K, Wang Q, Li Z, Shen J, Li T, Wang M, Wen Z, Li W, Qiang Y, Wang T, Ji J, Wu N, Wang Z, Zhang B, Feng G, He L, Xu Y, Shi Y. Br J Psychiatry. 2016, 208(5):441-5.  

Ÿ   TCR-CD3ζ gene polymorphisms and expression profile in rheumatoid arthritis. Li P, Wang X, Zhao MQ, Li LJ, Zhang C, Li BZ, Liu J, Yang XK, Leng RX, Fan YG, Pan HF, Ye DQ. Autoimmunity. 2016, 49(7):466-471.

Ÿ   Genetic Polymorphism (rs329498) in the Pellino-1 Gene as Possible Predisposal Factor for Systemic Lupus Erythematosus in a Chinese Population. Ni J, Liu J, Leng RX, Pan HF, Ye DQ. Immunol Invest. 201628:1-10. 

Ÿ   Association between IRAK1 rs3027898 and miRNA-499 rs3746444 polymorphisms and rheumatoid arthritis : A case control study and meta-analysis. Yang XK, Li P, Zhang C, Leng RX, Li S, Liu J, Li BZ, Pan HF, Ye DQ. Z Rheumatol. 2016 Aug 31. 

Ÿ   Associations of genetic variations in EYA4, GRHL2 and DFNA5 with noise-induced hearing loss in Chinese population: a case- control study. Zhang X, Liu Y, Zhang L, Yang Z, Yang L, Wang X, Jiang C, Wang Q, Xia Y, Chen Y, Wu O, Zhu Y. Environ Health. 2015; 14: 77.

Ÿ   Association of UBASH3A gene polymorphisms and systemic lupus erythematosus in a Chinese population. Liu J, Liu J, Ni J, Leng RX, Pan HF, Ye DQ. Gene. 2015, 565:116-21. 

Ÿ   Genetic variations in protocadherin 15 and their interactions with noise exposure associated with noise-induced hearing loss in Chinese population. Zhang X, Liu Y, Zhang L, Yang Z, Shao Y, Jiang C, Wang Q, Fang X, Xu Y, Wang H, Zhang S, Zhu Y. Environ Res. 2014;135:247-52.

Ÿ   Refined association of TSH receptor susceptibility locus to Graves' disease in the Chinese Han population.

Ÿ   Liu BL, Yang SY, Liu W, Xue LQ, Chen X, Pan CM, Gu ZH, Zhan M, Zhang XM, Liang J, Gao GQ, Du WH, Yuan GY, Ying R, Zhao SX, Song HD. Eur J Endocrinol. 2014, 170(1):109-19.

Ÿ   A genome-wide association study identifies two new risk loci for Graves' disease. Chu X, et al., Nat Genet. 2011, 43:897-901.


2.  农业研究

Ÿ   A Complex Structural Variation on Chromosome 27 Leads to the Ectopic Expression of HOXB8 and the Muffs and Beard Phenotype in Chicken. Guo Y, Gu X, Sheng Z, Wang Y, Luo C, Liu R, Qu H, Shu D, Wen J, Crooijmans R, Carlborg Ö, Zhao Y, Hu X, Li N. PLoS Genet. 2016 Jun; 12(6): e1006071.  

Ÿ   Development and application of absolute quantitative detection by duplex chamber-based digital PCR of genetically modified maize events without pretreatment steps. Zhu P, Fu W, Wang C, Du Z, Huang K, Zhu S, Xu W. Anal Chim Acta. 2016 Apr 15;916:60-6. 

Ÿ   A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment. Fu W, Zhu P, Wang C, Huang K, Du Z, Tian W, Wang Q, Wang H, Xu W, Zhu S, Sci Rep. 2015; 5: 12715.    

Ÿ   Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples. Li L, Zhang X, Wan Y, and Jin W. Biomed Res Int. 2013: 134675   


3.  干细胞研究         

Ÿ   A molecular roadmap for induced multi-lineage trans-differentiation of fibroblasts by chemical combinations. Han X , Yu H , Huang D, et al., Cell Research (2017) :1-16       

Ÿ   Improvement in Mouse iPSC Induction by Rab32 Reveals the Importance of Lipid Metabolism during Reprogramming. Pei Y, Yue L, Zhang W, Wang Y, Wen B, Zhong L, Xiang J, Li J, Zhang S, Wang H, Mu H, Wei Q, Han J. Sci Rep. 2015, 5: 16539.

Ÿ   Paracrine Action of Mesenchymal Stem Cells Revealed by Single Cell Gene Profiling in Infarcted Murine Hearts. Yao Y, Huang J, Geng Y, Qian H, Wang F, Liu X, Shang W, Nie S, Liu N, Du X, Dong J, Ma C. PLoS One. 2015, 10(6): e0129164.  

Ÿ   Single Cell Gene Profiling Revealed Heterogeneity of Paracrine Effects of Bone Marrow Cells in Mouse Infarcted Hearts. Li Y, Guo X, Xue Q, Zhu M, Gao L, Wang Y. PLoS One. 2013; 8(7): e68270. 

Ÿ   Parthenogenetic haploid embryonic stem cells produce fertile mice.Wan H, He Z, Dong M, Gu T, Luo G, Teng F, Xia B, Li W, Feng C, Li X, Li T, Shuai L, Fu R, Wang L, Wang X, Zhao X, Zhou Q. Cell Res. 2013, 23(11): 1330–1333.        


4.  肿瘤研究 

Ÿ   Diverse in vivo effects of soluble and membrane-bound M-CSF on tumor-associated macrophages in lymphoma xenograft model. Liao J, Feng W, Wang R, Ma S, Wang L, Yang X, Yang F, Lin Y, Ren Q, Zheng G. Oncotarget. 2016, 7(2): 1354–1366.      

Ÿ   Leukemic marrow infiltration reveals a novel role for Egr3 as a potent inhibitor of normal hematopoietic stem cell proliferation. Cheng H, Hao S, Liu Y, Pang Y, Ma S, Dong F, Xu J, Zheng G, Li S, Yuan W, Cheng T. Blood. 2015, 10; 126(11): 1302–1313. 

Ÿ   Diffuse large B-cell lymphoma: sub-classification by massive parallel quantitative RT-PCR. Xue X, Zeng N, Gao Z, Du MQ. Lab Invest. 2015;95(1):113-20. 


5.  液态活检 

Ÿ   Quantification and Dynamic Monitoring of EGFR T790M in Plasma Cell-Free DNA by Digital PCR for Prognosis of EGFR-TKI Treatment in Advanced NSCLC. Wang Z, Chen R, Wang S, Zhong J, Wu M, Zhao J, Duan J, Zhuo M, An T, Wang Y, Bai H, Wang J. PLoS One. 2014; 9(11): e110780. 


6.  生殖发育 

Ÿ   Endocardium Minimally Contributes to Coronary Endothelium in the Embryonic Ventricular Free Walls. Zhang H, Pu W, Li G, Huang X, He L, Tian X, Liu Q, Zhang L, Wu SM, Sucov HM, Zhou B. Circ Res. 2016 Jun 10;118(12):1880-93.  

Ÿ   Spatial Transcriptome for the Molecular Annotation of Lineage Fates and Cell Identity in Mid-gastrula Mouse Embryo. Peng G, Suo S, Chen J, Chen W, Liu C, Yu F, Wang R, Chen S, Sun N, Cui G, Song L Tam PP, Han JD, Jing N. Dev Cell. 2016, 36(6):681-97. 

Ÿ   Knockdown of p66Shc by siRNA injection rescues arsenite-induced developmental retardation in mouse preimplantation embryos. Ren K, Li X, Yan J, Huang G, Zhou S, Yang B, Ma X, Lu C. Reprod Toxicol. 2014, 43:8-18.   


7.  生物标记物筛选 

Ÿ   Microfluidic chip-based technologies: emerging platforms for cancer diagnosis. Ying L and Wang Q. BMC Biotechnol. 2013; 13: 76. 


8.  遗传病研究

     Association of long noncoding RNAs expression levels and their gene polymorphisms with systemic lupus erythematosus. Li, J., Wu, G.C., Zhang, T.P. et al. Sci Rep. (2017): 15,119


9.  生物信息学         

Ÿ   Revealing Missing Human Protein Isoforms Based on Ab Initio Prediction, RNA-seq and Proteomics. Hu Z, Scott SH, Qin G, Zheng G, Chu X, Xie L, Adelson LD, Oftedal EB, Venugopal P, Babic M, Hahn NC, Zhang B, Wang X, Li N, Wei C. Sci Rep. 2015; 5: 10940.    


10.          方法学研究  

Ÿ   Accurate quantification of supercoiled DNA by digital PCR. Dong L, Yoo HB, Wang J, Park SR. Sci Rep. 2016;6:24230.  

Ÿ   Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material. Dong L, Meng Y, Sui Z, Wang J, Wu L, Fu B. Sci Rep. 2015;5:13174.  



C1单细胞自动制备系统 + Biomark HD


1.      干细胞及发育

Ÿ   Generation and Application of Mouse-Rat Allodiploid Embryonic Stem Cells. Li X, Cui XL, Wang JQ, Wang YK, Li YF, Wang LY, Wan HF, Li TD, Feng GH, Shuai L, Li ZK, Gu Q, Hao J, Wang L, Zhao XY, Liu ZH, Wang XJ, Li W, Zhou Q. Cell. 2016, 164(1-2):279-92.      

Ÿ   Serum-Based Culture Conditions Provoke Gene Expression Variability in Mouse Embryonic Stem Cells as Revealed by Single-Cell Analysis. Guo G, Pinello L, Han X, Lai S, Shen L, Lin TW, Zou K, Yuan GC, Orkin SH. Cell Rep. 2016;14(4):956-65.  

Ÿ   Random X-chromosome inactivation dynamics in vivo by single-cell RNA sequencing. Wang M, Lin F, Xing K, Liu L. BMC Genomics. 2017, 18(1):90.   

Ÿ   Systematic Reconstruction of Molecular Cascades Regulating GP Development Using Single-Cell RNA-Seq. Li J, Luo H, Wang R, Lang J, Zhu S, Zhang Z, Fang J, Qu K, Lin Y, Long H, Yao Y, Tian G, Wu Q. Cell Rep. 2016;15(7):1467-80.


2.      干细胞及神经生物学

Ÿ   Direct Conversion of Normal and Alzheimer's Disease Human Fibroblasts into Neuronal Cells by Small Molecules. Hu W, Qiu B, Guan W, Wang Q, Wang M, Li W, Gao L, Shen L, Huang Y, Xie G, Zhao H, Jin Y, Tang B, Yu Y, Zhao J, Pei G.

Ÿ   Cell Stem Cell. 2015, 17(2):204-12. 


3.      骨科疾病研究

Ÿ   Single-cell analysis reveals a nestin+ tendon stem/progenitor cell population with strong tenogenic potentiality.Yin Z, Hu JJ, Yang L, Zheng ZF, An CR, Wu BB, Zhang C, Shen WL, Liu HH, Chen JL, Heng BC, Guo GJ, Chen X, Ouyang HW. Sci Adv. 2016;2(11):e1600874.



4.      肾脏疾病研究

Ÿ   Single-cell RNA-sequence analysis of mouse glomerular mesangial cells uncovers mesangial cell essential genes. Lu Y, Ye Y, Yang Q, Shi S. Kidney Int. 2017 Mar 17.





Ÿ   The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells. Geng J, et al., Nature Immunology. (2017) DOI10.1038/ni.3748