A Deep Profiler’s Guide to Cytometry
Bendall, S.C., Nolan, G.P., Roederer, M., Chattopadhyay, P.K.In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes in individual cells. The ability to simultaneously measure multiple genes on the same cell is necessary to resolve the incredible diversity of cell subsets, as well as to define their function in the host. Fluorescence-based flow cytometry is the benchmark for this; with it, we can quantify 18 proteins per cell, at >10,000 cells per second. “Mass cytometry” is a new technology that promises to significantly extend these capabilities. Immunophenotyping by mass spectrometry provides the ability to measure more than three dozen proteins at a rate of 1,000 cells per second. We review these cytometric technologies, capable of high-content, high-throughput single-cell assays.
Bendall, S.C., Nolan, G.P., Roederer, M., Chattopadhyay, P.K. "A Deep Profiler’s Guide to Cytometry" Trends in Immunology (2012): 323–32