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Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells

Polikowsky, H.G., Wogsland, C.E., Diggins, K.E. et al.

Differences in the quality of BCR signaling control key steps of B cell maturation and differentiation. Endogenously produced H2O2 is thought to fine tune the level of BCR signaling by reversibly inhibiting phosphatases. However, relatively little is known about how B cells at different stages sense and respond to such redox cues. In this study, we used phospho-specific flow cytometry and high-dimensional mass cytometry (CyTOF®) to compare BCR signaling responses in mature human tonsillar B cells undergoing germinal center (GC) reactions. GC B cells, in contrast to mature naive B cells, memory B cells, and plasmablasts, were hypersensitive to a range of H2O2 concentrations and responded by phosphorylating SYK and other membrane-proximal BCR effectors in the absence of BCR engagement. These findings reveal that stage-specific redox responses distinguish human GC B cells. 

Citation

Polikowsky, H.G., Wogsland, C.E., Diggins, K.E. et al. "Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells" Journal of Immunology (2015): 1,364–7