Expanding the clinical cytometry toolbox—Receptor occupancy by mass cytometry
Huse, K.Determination of the optimal therapeutic dose is a key challenge in drug development. Receptor occupancy assays (RO assays) measure the binding of biotherapeutics to their cellular targets and are one of the tools used to determine starting doses in clinical and preclinical studies. Flow cytometry‐based RO assays have been developed for heterogeneous cell populations in suspension and are well suited for evaluating therapeutics targeting peripheral blood cells. Mass cytometry would further expand the possibilities to measure receptor occupancy of complex cell types as metal‐labeled antibodies and minimal overlap between channels allow for more than 40 markers to be detected simultaneously without loss of sensitivity. Bringeland et al. 1 have developed a mass cytometry‐based RO assay, recently described in a technical note. With a backbone of 15 lineage markers, they identified eight cell types of interest and obtained receptor occupancy data for each cell population.
Huse, K. "Expanding the clinical cytometry toolbox—Receptor occupancy by mass cytometry" Cytometry Part A (2019): doi: 10.1002/cyto.a.23784