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High throughput gene expression measurement with real time PCR in a microfluidic dynamic array

Spurgeon, S.L., Jones, R.C., Ramakrishnan, R.

We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

Citation

Spurgeon, S.L., Jones, R.C., Ramakrishnan, R. "High throughput gene expression measurement with real time PCR in a microfluidic dynamic array" PLoS One (2008): e1662