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ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers

Leipold, M.D., Herrera, I., Ornatsky, O. et al.

Lectins have been increasingly important in the study of glycoproteins. Here, we report a glycoprofiling method based on the covalent attachment of metal-chelating polymers to lectins for use in an ICP-MS-based assay. The labeled lectins are able to distinguish between glycoproteins covalently attached to a microtiter plate and their binding can be directly quantified by ICP-MS. Since each conjugate contains a different lanthanide, the assays can be conducted in a single or multiplex fashion, and may be readily elaborated to many different assay formats.

Citation

Leipold, M.D., Herrera, I., Ornatsky, O. et al. "ICP-MS-based multiplex profiling of glycoproteins using lectins conjugated to lanthanide-chelating polymers" Journal of Proteome Research (2009): 443–9