Live cell barcoding for efficient analysis of small samples by mass cytometry
Wagar, L.E.In mass cytometry, sample loss is of considerable concern due to the relative inefficiency of cell event collection compared to similar techniques such as flow cytometry. Cell stimulation and the harsh conditions required in the later stages of certain sample preparations also contribute to cell loss. Low starting cell numbers are especially susceptible to these effects, potentially limiting the ability to use mass cytometry. Here is presented a live cell barcoding scheme and additional efficiency methods to improve recovery and achieve consistent staining for small samples.
Wagar, L.E. "Live cell barcoding for efficient analysis of small samples by mass cytometry" Mass Cytometry: Methods and Protocols (2019): 125-135