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Mass cytometry moving forward in support of clinical research: advantages and considerations

Nassar, A.F., Wisnewiski, A.V., Raddassi, K.

Since 1970, the invention of flow cytometry FACS has become widely used for single-cell measurements in medicine and biology. In the last 30 years FACS has become the work horse to provide fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. A recent breakthrough, cytometry time-of-flight (CyTOF®, mass cytometry), builds on flow cytometry and adds MS for unprecedented marker detection in biological samples. CyTOF is a novel and exciting technology for real-time analysis of single cells using inductively coupled plasma TOF-MS. Current CyTOF technique allows the detection of 40 parameters at the single-cell level; this capacity will increase as more isotopes become available. CyTOF can resolve multiple metal conjugated antibodies for biomarker detection on cells with minimal signal overlap, which maximizes the information obtained from each individual sample. To make the best use of mass cytometry to produce high-quality quantitative data from clinical samples, we must understand the experimental variables and how to minimize them.

Citation

Nassar, A.F., Wisnewiski, A.V., Raddassi, K. "Mass cytometry moving forward in support of clinical research: advantages and considerations" Bioanalysis (2016): 255–7