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Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut

Tordesillas, L., Rahman, A.H., Hartmann, B.M. et al.

Anaphylaxis is a serious, life-threatening immediate hypersensitivity reaction that is caused by cross-linking of IgE bound to FcεRI on mast cells and basophils. However, it has been described that anaphylaxis can also be induced by alternative pathways, mediated by IgG-antigen immune complexes, FcγRIII, and basophils, neutrophils, and/or macrophages. The availability of human data regarding the acute response to allergens by different leukocyte populations in allergic patients is scarce. Mass cytometry uses heavy metal–labeled antibodies and time of flight mass spectrometry for detection, allowing for simultaneous detection of more than 40 markers. This multiplexing capacity allows for simultaneous profiling of all hematopoietic cells, and provides a unique platform with which to comprehensively study the human immune response to allergens.

Citation

Tordesillas, L., Rahman, A.H., Hartmann, B.M. et al. "Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut" Journal of Allergy and Clinical Immunology (2016): 1741–4.e9