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Multiplex MHC class I tetramer combined with intranuclear staining by mass cytometry

Simoni, Y., Fehlings, M., and Newell, E.W.

Antigen-specific CD8+ T cells play a crucial role in the host protective immune response against viruses, tumors, and other diseases. Major histocompatibility complex (MHC) class I tetramers allow for a direct detection of such antigen-specific CD8+ T cells. Using mass cytometry together with multiplex MHC class I tetramer staining, we are able to screen more than 1000 different antigen candidates simultaneously across tissues in health and disease, while retaining the possibility to deliver an in-depth characterization of antigen-specific CD8+ T cells and associated phenotypes. Here we describe the method for a MHC class I tetramer multiplexing approach together with intracellular antibody staining for a parallel phenotypic cell characterization using mass cytometry in human specimens.

Citation

Simoni, Y., Fehlings, M., and Newell, E.W. "Multiplex MHC class I tetramer combined with intranuclear staining by mass cytometry" Mass Cytometry: Methods and Protocols (2019): 147–58.