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Multiplexed peptide-MHC tetramer staining with mass cytometry

Leong, M.L., Newell, E.W.

Mass cytometry is flow cytometry based on single cell mass spectrometry with decreased crosstalk between channels and an ability to probe >40 parameters per cell, making it well suited for multiplexed assays. Peptide major histocompatibility (MHC) tetramer staining allows direct detection of antigen specific cells and is also amenable to multiplexing/combinatorial approaches. Here we describe methods for multiplexed pMHC-tetramer staining using mass cytometry.

Citation

Leong, M.L., Newell, E.W. "Multiplexed peptide-MHC tetramer staining with mass cytometry" Methods in Molecular Biology (2015): 115–31