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Publications

OMIP-048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry

Jaracz-Ros, A., Hémon, P., Krzysiek, R. et al.

Calcium (Ca2+) signaling controls T-cell activation and functions. Ca2+ concentrations are locally detected and controlled by Ca2+-sensors (STIM1 and 2 detecting the depletion from ER stores channels) and Ca2+-channels (ORAI1-3 in the cell membrane and VDAC1 in the outer mitochondrial membrane). We first validated and titrated antibodies to assess the expression of these Ca2+-sensors and -channels in human and murine cells, and further devised a 18-antibodies mass cytometry panel to characterize their expression in primary murine lymphocyte subsets.

Citation

Jaracz-Ros, A., Hémon, P., Krzysiek, R. et al. "OMIP-048 MC: Quantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometry" Cytometry Part A: Journal of the ISAC (2018): 681–4