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Significant interference in mass cytometry from medicinal iodine in human lung

Keller, B.C., Presti, R.M., Byers, D.E. et al.

The use of mass cytometry (inductively coupled plasma mass spectroscopy performed on rare metal-labeled single cells) has significantly expanded the ability to identify unique cell subsets in samples based on the multiplicity of surface markers that can be queried simultaneously. Traditional flow cytometry on lung specimens, particularly alveolar macrophages, is significantly hindered by autofluorescence that limits the number of useful channels and dynamic range of cell protein evaluation. In addition, macrophage autofluorescence is enhanced by common environmental exposures, such as smoke, degrading the ability to compare samples from smoking and nonsmoking subjects. Mass cytometry overcomes these limitations by using rare metal isotopes covalently coupled to antibodies, rather than fluorophores. For this reason, mass cytometry has incredible potential for defining novel macrophage subsets in human samples. A wide range of rare metal tags and lack of interference from freezing or fixation means existing small-quantity samples banked from prior studies or clinical trials can produce multidimensional data for novel analysis of macrophage subsets.

Citation

Keller, B.C., Presti, R.M., Byers, D.E. et al. "Significant interference in mass cytometry from medicinal iodine in human lung" American Journal of Respiratory Cell and Molecular Biology (2016): 150-151