日本

Publications

Staining of phosphorylated signalling markers protocol for mass cytometry

Shinko, D., Ashhurst, T.M., McGuire, H.M. et al.

Mass cytometry is a multi-parametric technique that offers insight into functional and biological systems at a single cell level. One of the major advantages of mass cytometry is the ability to measure multiple intracellular markers, including phosphorylated proteins that are part of major signaling pathways, such as NF-κB, JAK/STAT, and ERK/MAPK. Here we describe an optimized mass cytometry protocol for staining human clinical blood samples with panels that include phosphorylated antibodies.

Citation

Shinko, D., Ashhurst, T.M., McGuire, H.M. et al. "Staining of phosphorylated signalling markers protocol for mass cytometry" Mass Cytometry: Methods and Protocols (2019): 139-146