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Publications

T cell fate and clonality inference from single-cell transcriptomes

Stubbington, M.J.T., Lönnberg, T., Proserpio, V. et al.

We developed TraCeR, a computational method to reconstruct full-length, paired T cell receptor (TCR) sequences from T lymphocyte single-cell RNA sequence data. TraCeR links T cell specificity with functional response by revealing clonal relationships between cells alongside their transcriptional profiles. We found that T cell clonotypes in a mouse Salmonella infection model span early activated CD4+ T cells as well as mature effector and memory cells.

Citation

Stubbington, M.J.T., Lönnberg, T., Proserpio, V. et al. "T cell fate and clonality inference from single-cell transcriptomes" Nature Methods (2016): 329–32