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Titration of mass cytometry reagents

van Vreden, C., Niewold, P., McGuire, H.M. et al.

Mass cytometry (MC) is a powerful research tool enabling high-dimensional analysis of single cells in suspension and within tissue sections following laser ablation. Here we describe the procedure of titrating metal-conjugated antibodies, to ensure that optimal levels of staining are achieved while minimizing nonspecific signals that may occur at high concentrations.

Citation

van Vreden, C., Niewold, P., McGuire, H.M. et al. "Titration of mass cytometry reagents" Mass Cytometry: Methods and Protocols (2019): 83-92