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Visualization of mass cytometry signal background to enable optimal core panel customization and signal threshold gating

Au-Yeung, A., Takahashi, C., Mathews, W.R. et al.

Signal interference or overlap in mass cytometry is minimal compared to flow cytometry but must still be considered for optimal panel design and assay sensitivity. Here we describe a procedure for evaluating signal interference dynamics in the context of a 25-parameter core immunophenotyping panel. Specifically, a mass-minus-many (MMM) approach was used to assess background signals in “empty” or “blank” channels intended for further customization. Through this approach cell type-specific variability in signal background is revealed. Further panel customization can thus be performed with an understanding of cell type and channel-specific background levels to enable rational panel design and the objective delineation of gating thresholds during analysis.

Citation

Au-Yeung, A., Takahashi, C., Mathews, W.R. et al. "Visualization of mass cytometry signal background to enable optimal core panel customization and signal threshold gating" Mass Cytometry: Methods and Protocols (2019): 35-45