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Publications

WS10: Best practices for development and implementation of a CyTOF® core

Rayanki,R., Ashhurst,T.M., van Vreden, C. et al.

Mass cytometry promises to be a potential game changing technology that provides unmatched depth of immune system characterization at the single cell level. The method dramatically expands the capability of cell subset identification and phenotypic characterization compared to flow cytometry (FCM). The use of rare‐earth metal isotopes conjugated to monoclonal antibodies results in a minimal overlap between channels and enables seamless evaluation of multiple parameters simultaneously on individual cells. The difficulties in this platform typically concern 1. ease‐of‐use, 2. high cost, 3. creation of large panels, and 4. data analysis and visualization, all of which can be limiting factors or roadblocks to the widespread implementation and use of this technology compared to flow cytometry.

The workshop was held to aid flow cytometry labs who are seeking to establish mass cytometry capability in their lab, but may have struggled with implementation due to any number of unforeseen challenges. We intended to discuss these challenges and adapted solutions from experts in the field who both operate flow and mass cytometry cores.

Citation

Rayanki,R., Ashhurst,T.M., van Vreden, C. et al. "WS10: Best practices for development and implementation of a CyTOF® core" Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops (2019): 619–621.