How much RNA is needed for the reverse transcriptase and preamplification steps of RT-PCR?
For Fluidigm Reverse Transcription Master Mix, we recommend from 150 ng/μL down to 1 pg/μL of total RNA as starting material. For Fluidigm Preamplification Master Mix, we recommend the cDNA equivalent of 12.5 ng/μL down to <0.25 pg/μL of total RNA.
For Research Use Only. Not for use in diagnostic procedures.