The Counter Culture
For too long differentiation and reprogramming have meant being resigned to long manual hours, weekends at the bench and frustrating, inconsistent results. But what if you could choose a different fate—for you and your cells? Introducing Callisto™, the industry-first platform that lets you design, optimize and execute multistage, long-term protocols with a few taps on a screen. Program your experiment offline, collaborate on protocols and, when you’re ready, press Run and walk away... for up to three weeks.
Optimize your experiment with multifactorial dosing of your cells with any combination of RNA, small molecules, virus and other differentiation factors.
Save time, effort and cost with an easy workflow based on prescheduled media and dosing changes and nanoliter-scale reaction volumes.
Accelerate discovery with integrated workflows for live-cell, RNA, DNA or protein analysis.
Run a complete culture protocol within a single instrument with just a touch of your finger.
Callisto revolutionizes cell model studies with a new, fully integrated system for precise manipulation of biological pathways for in vitro cell culture. The system’s innovative integrated fluidic circuit (IFC) includes 32 independent chambers that can be controlled for up to three weeks with minimal intervention. Design protocols anywhere using the drag-and-drop features of the Callisto Experiment Planner to schedule feeding, dosing and harvesting, and monitor experiments remotely, for a more efficient workflow than ever before possible. When your protocol is done, you can harvest single-cell suspensions or bulk cells to prepare for the analysis of your choice, including targeted gene expression, whole transcriptome, microRNA analysis, DNA sequencing, imaging or live-cell plating and expansion.
Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.
IFCs, Assays and Reagents
Callisto™ Lysis Reagent Kit
Prepare your cells with this all-in-one kit enabling downstream RNA, DNA or protein analysis.
Callisto™ Reagent Kit
Simplify cell loading and multi-stage cell culture maintenance with our kit optimized for Callisto.
Callisto System Software v3.8.13
The latest operating system upgrade for the Callisto™ system. To install, follow the instructions found in the Callisto System User Guide (in Chapter 3, see Update the System).
Callisto Experiment Planner v2.0.1
Easily design and create intricate multifactorial dosing, feeding and harvesting protocols for your cell modeling experiment on Callisto. Save and share protocols with colleagues to ensure reproducible and reliable cell culture results.
Dimensions as Shipped
|Height||32.5 in (82.6 cm)|
|Width||26.2 in (66.5 cm)|
|Depth||38.6 in (98.0 cm)|
|Weight||180 lb (82 kg)|
|Height||19.7 in (50.0 cm)|
|Width||17.3 in (43.9 cm)|
|Depth||27.5 in (69.8 cm)|
|Weight||137 lb (62 kg)|
|Clearance||4 in (10 cm) from the rear, 10 in (25 cm) from the front|
|Temperature||Ambient between 15 and 28ºC (59 to 82ºF) stable|
|Premixed Gas Requirements||Clean, dry, premixed gas containing 5% CO2, 5–22% O2, balanced by N2|
|Premixed Gas Regulation||22–25 psi (152–172 kPa)|
|Compressed Air Requirement||75–90 psi (517–621 kPa)|
|Altitude Site Preparation||<8,202 ft (2,500 m)|
Refer to the Callisto Site Requirements (PN 100-7601) for more information on operating systems.
|Thermal Control||Peltier-based, 4–39 ºC ± 1 ºC|
|Max Heating Rate||>4ºC/sec|
|Max Cooling Rate||>3ºC/sec|
|Humidity||90 ± 5%|
|IFC Compatibility||Callisto IFCs for Adherent Cell Culture|
|Current||8.0 A (max)|
|Main Supply Fluctuation||Not to exceed ±10% of the nominal supply voltage|