Japan-News

Polaris

Seamless integration of cell biology and molecular analysis at the single-cell level

Product

The Future is Functional Genomics

Imagine if you could explore the genomes of every cell to find the gene networks that regulate them. You could correlate a cell’s phenotype, physiological condition and molecular signature to discover the genetic circuits regulating cell function or compare a population’s sensitivity to environmental changes. It’s the future of single-cell biology, and it’s available now on Polaris™, the first system to seamlessly integrate cell and molecular biology at the single-cell level. Select individual cells, challenge them with factors you choose under strict environmental conditions for up to 24 hours and measure each cell’s acute response transcriptionally via next-generation sequencing. And because it all happens on one precisely designed IFC, controlled by an intuitive touchscreen interface, all that’s left to the imagination is what question you’ll answer next. 

Precise

Select your target cell by phenotype, viability or transgenic reporter.

Seamless

Select, dose and prepare cells for analysis in one integrated workflow.

Interactive

Observe and manipulate cells in their biological environment.

Flexible

Challenge cells with a variety of parameters with the tap of a touchscreen.

テクノロジー

An innovative integrated fluidic circuit (IFC) enables Polaris to actively select single cells from heterogeneous cell samples and hold them in individual, environmentally controlled reaction chambers. Once isolated, cells can be challenged with a variety of factors, including transcription factors, RNAs and small molecules, at the dosage or intervals you define. Then the cells are automatically lysed and RNA template is prepared for mRNA sequencing analysis. Polaris does it all in a single IFC and with minimal hands-on time, controlled by an elegant, intuitive touchscreen interface.

ワークフロー

  1. Prime
  2. Transfer
  3. Load
  4. Dose
  5. Lyse
  6. Harvest

Prime

Prime the Polaris IFC with extracellular matrix and buffers to prepare it for sample transfer.

Transfer

Pipet live cells in suspension and reagents into the IFC.

Load

Insert the IFC into Polaris, which automatically images, selects and isolates individual cells according to your specifications.

Dose

Challenge selected cells with a variety of parameters and stain, if desired.

Lyse

Lyse each cell to reverse transcribe and amplify within the IFC.

Harvest

Collect the product from the harvest output for library prep and sequencing.

Support

Polaris Support

Browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here

構成

IFCs, Assays and Reagents

Polaris IFC

Select, perturb and observe transcriptional changes in individual cells with the Polaris™ IFC. Our most sophisticated microfluidic IFC integrates cell biology and genomic analysis into one workflow so you can find the gene networks that regulate each cell. Designed for use with the Polaris system.

  1. Polaris IFC

関係する製品

Biomark™ HD

Biomark™ HD Biomark HDシステムを使用し、シングルセルの遺伝子発現をリアルタイムPCRで解析することで、業界最高のフレキシビリティと共に一貫して高品質な結果を得ることができます。

C1™

C1™ 高精度でありながらフレキシブルな単一ワークフローで、シングルセルの単離、染色、溶解、および増幅を行います。革新的なC1 Single-Cell Auto Prepシステムにより、全てのステップがわずか4時間で終了します。
  1. Biomark™ HD
  2. C1™

ソフトウェア

Singular解析

 

  1. Singular解析

仕様

Dimensions as Shipped

Height 42.0 in (106.8 cm)
Width 28.6 in (72.7 cm)
Depth 37.3 in (94.8 cm)
Weight 300 lb (136 kg)

Uncrated

Height 27.8 in (70.6 cm)
Width 20.6 in (52.2 cm)
Depth 34.1 in (86.6 cm)
Weight 242 lb (110 kg)

Site Requirements

Clearance 6 in (15 cm) from the rear
Temperature 15–28 ºC (59–82 ºF) stable
Humidity 30–80%, noncondensing
Altitude site preparation <8,202 ft (2,500 m)

Components

Current 10 A (max)
Voltage 90 V (min) to 240 V (max)
Frequency 50/60 Hz
Main supply fluctuation not to exceed ±10% of the nominal supply voltage
Connection 4x USB 2.0
Humidity 30–80%, noncondensing

Gas Requirements

Grade Clean, dry premixed gas (standard grade)
Blood gas 5% CO2, 5% oxygen and 90% nitrogen
Mixed gas 5% CO2, 20% oxygen and 75% nitrogen
Regulatory 22–25 psi