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Advanta™ RNA-Seq XT NGS Library Prep Kit (101-9950)

This kit uses a polyadenylated [poly(A)] enrichment approach to prepare NGS-ready, full-length cDNA libraries from total RNA. The kit contains sufficient 48.Atlas™ IFCs (4 per kit), reagents, Control Line Fluid, and unique dual indexes (UDIs) to generate libraries for 192 samples, and it includes 4 sets of 1–48 UDI barcodes.


Kit Contents

  • 4 IFCs—48.Atlas (101-9303)
  • 8 syringes—Control Line Fluid (101-9307, 150 μL each)
  • Reagents in four boxes: Module 1, Module 2, Module 3A, and Module 4

Advanta RNA-Seq NGS Library Prep Kit, Module 1: (–80 °C, 101-9299)

  • TSO Mix—1 tube with 50 μL (101-9091)

Advanta RNA-Seq NGS Library Prep Kit, Module 1: (–20 °C, 101-9223)

  • 1 tube DNA Polymerase—130 μL (101-9096)
  • 1 tube Enhancer Buffer—100 μL (101-9101)
  • 2 (of 4) bottles of Harvest Reagent Type 1—3.7 mL each (101-9300)
  • 1 tube Loading Reagent Type 1—200 μL (101-9301)
  • 1 tube mRNA Binding Buffer—1 mL (101-9079)
  • 1 tube N6—15 μL (101-9092)
  • 1 bottle PCR Buffer—3.5 mL (101-9097)
  • 1 tube PCR2 Primer—65 μL (101-9099)
  • 1 tube of Post-Capture Buffer—1 mL (101-9081)
  • 1 tube of Pre-Capture Buffer—120 μL (101-9080)
  • 1 tube qPCR Primer Premix—70 µL (101-9755)
  • 1 tube RNase Inhibitor—20 μL (101-9095)
  • 1 tube RT Buffer—50 μL (101-9093)
  • 1 tube RT Enzyme—24 μL (101-9094)
  • 1 tube Tris Buffer—1.25 mL (101-9098)

Advanta RNA-Seq Unique Dual Index, Module 3A: (–20 °C, 101-9949)

  • 4 plates UDI-PCR Mix Plate A (1–48 index mixes per 48-well plate)—10 μL per well (101-9954)

Advanta RNA-Seq NGS Library Prep Kit, Module 4: (4 °C, 101-9308)

  • RNA Seq Beads—150 μL (101-9076)


Product insert available.


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