Japan-News

Product

消耗品

Maxpar® Human Immune Monitoring Panel Kit

The Maxpar Human Immune Monitoring Panel Kit is designed and optimized for deep immune profiling of human peripheral blood mononuclear cells (PBMC). The 29 antibodies in the kit enable the comprehensive identification and characterization of key immune cell populations, including all major T cell subsets (CD4+ and CD8+ naive, central memory, effector, and effector memory), CD4+ regulatory T cells, B cell subsets (naive, memory, and transitional), plasmablasts, natural killer (NK) cells, T helper (Th) cell subsets (Th1, Th2, and Th17), gamma delta (gd) T cells, monocytes, dendritic cells (plasmacytoid dendritic cells and myeloid dendritic cells), and granulocytes.

The panel kit is designed for use with the Helios™ system or a CyTOF® 2-to-Helios system.

Maxpar Human Immune Monitoring Panel

Target

Clone

Metal

CD45  HI30 89Y
CD196/CCR6  G034E3 141Pr
CD19 HIB19 142Nd
CD127/IL-7Ra  A019D5 143Nd
CD38 HIT2 144Nd
IgD IA6-2 146Nd
CD11c Bu15 147Sm
CD16 3G8 148Nd
CD194/CCR4  L291H4 149Sm
CD123/IL-3R 6H6 151Eu
TCRgd  11F2 152Sm
CD185/CXCR5  RF8B2 153Eu
CD3 UCHT1 154Sm
CD45RA  HI100 155Gd
CD27  L128 158Gd
CD28  CD28.2 160Gd
CD66b  80H3 162Dy
CD183/CXCR3 G025H7 163Dy
CD161  HP-3G10 164Dy
CD45RO UCHL1 165Ho
CD24  ML5 166Er
CD197/CCR7 G043H7 167Er
CD8  SK1 168Er
CD25/IL-2RA 2A3 169Tm
CD20 2H7 171Yb
HLA-DR L243 173Yb
CD4 SK3 174Yb
CD14 M5E2 175Lu
CD56  NCAM16.2 176Yb

Kit Contents:

  • Maxpar Metal-Conjugated Antibodies (29 markers, individually packaged)
  • Maxpar Cell Acquisition Solution
  • Maxpar Cell Staining Buffer
  • Maxpar Fix and Perm Buffer
  • Maxpar PBS
  • Cell-ID™ Intercalator–Ir
  • Cell-ID Cisplatin

Fluidigm products required but not included:

  • EQ™ Four Element Calibration Beads (Cat. No. 201078, 100 mL)
  • Tuning Solution (Cat. No. 201072, 250 mL)
  • WB Injector (Cat. No. 107950)*

*One free WB Injector is shipped per Helios instrument installed. Additional WB Injectors are available for purchase.

Reagents required from other vendors:

  • Human TruStain FcX™ (Fc-Receptor Blocking Solution) (BioLegend® Cat. No. 422301, 50 tests; Cat. No. 422302, 200 tests)
  • Pierce™ 16% Formaldehyde (w/v), Methanol-free (Thermo Fisher Scientific™ Cat. No. 28906 (10 x 1 mL); Cat. No. 28908 (10 x 10 mL)
  • Species:
    Human