ERK1 and ERK2, also known as p44 and p42 MAPKs, are similar (85% sequence identity) members of the mitogen-activated protein kinase (MAPK) family of serine/threonine protein kinases. ERK1/2 signaling is important in the cellular response to a wide range of stimuli including growth factors, cytokines and mitogens. The signal cascade upstream of ERK1/2 typically begins with receptor tyrosine kinases phosphorylating members of the Raf family and other MAP kinase kinase kinases (MAP3Ks), which thereby activate MEK1 and MEK2, the MAP kinase kinases (MAPKKs) directly responsible for phosphorylation of ERK1 and ERK2. ERK1 and ERK2 are activated through phosphorylation of the activation loop residues Thr202/Tyr204 and Thr185/Tyr187, and dual phosphorylation is required for full activity. ERK1/2 can activate the RSK family of kinases in the cytoplasm and transcription factors including Elk-1 in the nucleus.
Anti-pERK1/2 [T202/Y204] (D13.14.4E)-171Yb—25 µg