Histone H3, the most widely modified of all 4 nucleosomal histones, has several phosphorylation sites on its N-terminal tail. H3 is phosphorylated on threonine 3, serine 10, serine 28, and threonine 32 when cells enter mitosis. Phosphorylation at serine 10 or 28 occurs in association with the induction of immediate-early (IE) genes and is part of the nucleosomal response downstream of the activation of the ERK1/2 or p38 MAPK pathways. Mitogen- and stress-activated protein kinases 1 and 2 (MSK1 and MSK2) are activated by either MAPK pathway and have been identified as the kinases mediating the nucleosomal response. As a downstream target of MAPK signaling pathways, H3 phosphorylation is a response to a vast array of extracellular stimuli including growth factors, stressors such as UV light, alcohol, and neurotransmitters. During mitosis, Aurora B and PP1 phosphorylate and dephosphorylate H3 serine 10 and serine 28, respectively.
Anti-pHistone H3 [S28] (HTA28)-175Lu—50 Tests
Target:pHistone H3 [S28]