1714 検索結果 for "Targeted gene expression"
Gene Expression
Data reproducibility is key to differential gene expression studies. The adaptable Biomark™ system supports as few as 36 datapoints and as many as 9,216 on a single Dynamic Array™ IFC (integrated fluidic circuit). Combining microfluidics technology with optimized reagents produces more results in less time. Fluidigm Reverse Transcription Master Mix, Preamp Master Mix and Delta Gene™ reagent kits handle a variety of samples affordably and without sacrificing quality.
Learn more about: Biomark Juno™
Learn more about reagents for: Gene expression Single-cell targeted gene expression Digital PCR
For support Biomark & EP1™ Juno
More search results for Gene Expression:
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faq
How is cDNA generated for targeted gene expression analysis on the C1 system?
The targeted gene expression application uses the Ambion Single Cell-to-Ct reagents to generate random hexamer-primed cDNA from total RNA. …
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faq
Can I select which miRNAs are amplified during miRNA expression analysis on the C1 system?
No. miRNA expression analysis uses Megaplex™ Primer Pools (Thermo Fisher Scientific) to amplify predefined pools of hundreds of specific miRNA species. …
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faq
Can I use Biomark HD to screen samples from mRNA seq harvest?
It is possible to use the Biomark HD system to screen C1 system mRNA seq harvest samples and visualize the levels of gene expression from each cell. …
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faq
Can I use Delta Gene assays for miRNA expression analysis on the C1 system?
No. miRNA expression products from the C1 system are compatible only with TaqMan miRNA Assays. …
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faq
How is cDNA generated for miRNA expression analysis on the C1 system?
The targeted gene expression application uses a combination of the Ambion Single Cell-to-Ct reagents, TaqMan MicroRNA Reverse Transcription reagents and Megaplex Primer Pools (Thermo Fisher Scientific) to generate cDNA corresponding to specific pools of miRNAs. …
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faq
What methods are supported by the Juno instrument?
Juno™ processes DNA and RNA samples using proprietary Fluidigm library preparation (LP) IFCs to generate targeted libraries for next-generation sequencing. Juno can also be used as a universal IFC controller for pre-processing all Fluidigm genotyping, gene expression, and digital PCR IFCs upstream of final processing and analysis on the Fluidigm Biomark™ HD or EP1™ systems. …
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consumable
Single-Cell Targeted Gene Expression
Discover population prevalence with multiplex real-time PCR and miRNA analysis …
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faq
If a cell is caught somewhere besides the intended capture site, will it be amplified?
Cells caught anywhere between the valves (see image) will be lysed and included in all subsequent reactions. …
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product insert
C1 Reagent Kit for Preamplification Product Insert
This kit is used to facilitate the capture of cells and performing preamplification on single cells or for targeted gene expression analysis.
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faq
How do I prepare my cells for the C1 IFC?
All cells are handled differently. …
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media
Targeted Single-Cell Transcription Factor Profiling Outperforms mRNA-seq
Poster presented at AACR 2017 …
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brochure
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Poster
Targeted Single-Cell Transcription Factor Profiling Outperforms mRNA-seq for In-Depth Characterization of Cancer Cells
Poster presented at AACR 2017
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User Guide
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faq
What types of analysis software do people usually use after your platform?
The Singular Analysis Toolset from Fluidigm provides a set of software functions for higher-level expression analysis using R. …
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resource
C1 System Software
The C1™ system supports DNA sequencing, mRNA sequencing, targeted gene expression, miRNA analysis and additional applications through the C1 Open App™ IFC (integrated fluidic circuit). Download the C1 system software update and then follow the instructions for updating the C1 system software.
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consumable
Genomics and Gene Expression
Enhance your productivity with high-performance solutions …
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technical note
Single-Cell Genomics in Immunology Technical Note
Gene expression profiling at the single-cell level is possible with both targeted and whole transcriptome approaches, such as qPCR and mRNA sequencing (mRNA seq).
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consumable
Advanta Assays and Kits
Combining microfluidics technology with optimized reagents produces high-quality results in less time. …
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Publications